Is PGS for me?
Your specialist may recommend PGS if:
- For male -
• you are over 35 and have a higher risk of having a baby with a chromosome problem (such as Down’s syndrome)
- • you have a family history of chromosome problems
- • you have a history of recurrent miscarriages
• you have had several unsuccessful cycles of IVF where embryos have been transferred, or
- • your sperm are known to be at high risk of having chromosome problems.
However, various studies have questioned whether or not PGS is effective at increasing the chance of having a live birth.
How does PGS work?
The procedure for PGS is usually as follows:
Step 1. You undergo normal IVF or ICSI treatment to collect and fertilise your eggs
- Step 3. A trained embryologist removes one or two of the cells (blastomeres) from the embryo.
- Step 5. One, two or three of the embryos without abnormal numbers of chromosomes
are transferred to the womb so that they can develop. Any remaining unaffected embryos
can be frozen for later use.
Step 2. The embryo is grown in the laboratory for two to three days until the cells have
divided and the embryo consists of about eight cells.
- Step 4. The chromosomes are examined to see how many there are and whether they are normal.
- Step 6. Those embryos that had abnormal chromosomes are allowed to perish or may be used for research (with your consent).
There are possible variations to this procedure and the trophectoderm biopsy technique can be used in some cases.
Testing at five to six days
It is possible that instead of removing and testing one or two cells from a two – three day old embryo,
some centres may allow the embryo to develop to five - six days, when there are 100-150 cells.
More cells can be removed at this stage without compromising the viability of the embryo, possibly
leading to a more accurate test.
Alternatively some centres may test eggs for chromosomal abnormalities before they
are used to create embryos. Polar bodies (small cells extruded by eggs as they mature) can be extracted and tested.
Comparative Genomic Hybridisation (CGH)
Clinics are now using a procedure called comparative genomic hybridisation (CGH) which allows
centres to test for abnormalities in all 23 chromosomes.
These abnormalities may or may not be of biological significance, but their presence will lower
the chance of finding suitable embryos for transfer.
What are the risks of PGS?
Some of the risks involved in PGS treatment are similar to those for conventional IVF.
Other problems unique to PGS treatment include:
• some embryos may be damaged by the process of cell removal
• possibility that no embryos are suitable for transfer to the womb after PGS.
It is important to understand that there is no guarantee against a miscarriage occurring even though
PGS has been carried out prior to embryo transfer.